Journal: The EMBO Journal
Article Title: Wnt and Src signals converge on YAP‐TEAD to drive intestinal regeneration
doi: 10.15252/embj.2020105770
Figure Lengend Snippet: Src family kinase inhibition via Dasatinib treatment in control organoids enforces YAP cytoplasmic localisation after 4 h of treatment in organoids cultured for 3 days. n > 10 organoids in each experiment. Quantification of the percentage of nuclear YAP cells per control crypt bud as shown in panel (A), error bars show 1 SD (**** P < 0.0001). n > 10 organoids per condition (top). Quantification of the nuclear/cytoplasmic ratio of YAP per cell in Apc5 mutant organoids as shown in panel (C). n > 10 organoids per condition (bottom). Statistical analysis was performed with an unpaired Student's t ‐test. Src family kinase inhibition via Dasatinib or eCF506 treatment in Apc5 organoids causes relocalisation of YAP to the cytoplasm. n > 10 organoids in each experiment. qPCR analysis of the YAP‐TEAD target gene Ctgf reveals a strong inhibition of mRNA expression upon treatment with eCF506 (relative expression, error bars = 1 SD). Statistical analysis was performed with an unpaired Student's t ‐test. Src family kinase inhibition via Dasatinib or eCF506 treatment in Villin‐Cre ERt Lats1 / 2 dKO organoids (induced in vitro with 4‐OHT) fails to cause relocalisation of YAP to the cytoplasm after 4 h treatment in culture. n > 10 organoids in each experiment. Source data are available online for this figure.
Article Snippet: The Porcupine inhibitor LGK974 (Selleck chemicals, S7143) was used at 5 μM for 24 h. For Src inhibition experiments, organoids were treated with 100 nM of Dasatinib (Selleck chemicals, S1021) or 100 nM eCF506 for a period of 4 h. Organoid microscopy was performed with either a Leica SP5 or a Leica SP8 laser‐scanning confocal microscope.
Techniques: Inhibition, Control, Cell Culture, Mutagenesis, Expressing, In Vitro
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